What does a negative real part of impedance indicate in a Nyquist plot?

I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...

12 January 2021 7,399 14 View

How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.

01 December 2020 1,099 2 View

What is the difference between the near field and the far field effects in optics?

A simple definition might help to understand the diffraction pattern in an aperiodic nano-micro structured surface.

19 July 2018 3,865 6 View

Is there a way to attach a nano particle to the cell membrane and prevent it from entering the cell?

01 June 2016 5,159 3 View

Will RGD coated nanoparticles be internalised by cells?

24 December 2015 6,303 4 View

Do we have "mesenchymal" breast cancer cell lines?

24 November 2015 8,669 6 View

What is the difference between various breast cancer cell lines?

24 November 2015 8,487 5 View

Is there any correlation between substrate stiffness and substrate roughness?

Can tuning roughness leads to different stiffness of a material?

18 October 2015 3,286 8 View

Is flow cytometry capable of calculating the viability rate of a low viscous cell solution?

As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether...

22 February 2013 5,130 2 View

Dual-fluorescence viability VS Trypan blue, which one is more accurate and why am I observing such a distinctive difference?

While encapsulating ADMSCs in sodium alginate, providing a 3D environment for the cells, I come across different viability rates. Trypan blue shows a lower viability rate, as low as 50%. On the...

19 February 2013 6,200 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View