I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip!
I swapped them with beads (similar size) instead and observed that 99% of the beads were stuck in the syringe. During the injection procedure, I assume the beads/organoids sediment in the syringe and stick to the plastic.
It seems like agitation is required during the injection procedure to prevent sedimentation, but that doesn't seem practical with the syringe pumps. Since I don't have many organoids available, I have to use every single one😊
It would be great to hear your thoughts, experiences, and recommendations!
It is useful to put small size magnets in the injector and add a rotating magnetic field around the injector. Alternatively, increasing the density or viscosity of the dispersion can be used to diminish particle settling. Good luck
Thanks Shitao Shen ! I heard about the magnetic thing; it sounds very smart!
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining