I am using a neutralized collagen and upon cross linkage in the incubator, the gel becomes opaque. Is there a way to make it perfectly transparent?
I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...
24 October 2023 7,385 0 View
Any suggestions for high quality miRNA extraction would be appreciated.
13 June 2023 5,958 3 View
Please help me. I don't know how can I design laparoscope with zemax.
01 April 2023 3,150 0 View
I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip! I swapped them with beads (similar size) instead and observed that...
26 February 2023 944 2 View
It's quite confusing to distinguish between the definitions. To me, autofluorescence fits all. But what is the correct terminology to use when talking about biological cells, proteins (such as...
14 February 2023 969 5 View
I am trying to immunostain whole (micro)tissue samples, and I think there is an antibody penetration issue. Would longer permeabilization procedures or harsher permeabilization agents help with...
14 February 2023 5,892 4 View
Hi, I fixed chondrocytes in 12-well plate with glutaraldehyde. I want to stain the cells by direct red. Do you have any protocol? Thank you.
04 February 2023 6,914 0 View
Hi, I performed RT-PCR directly on the osteoarthritic cartilages obtained from 2 patients. In both experiments, Ct for TNF-a was found to be around 36, which is unexpected considering the...
31 January 2023 7,269 0 View
I was wondering if it is possible to add a second photoinitiator to SU-8 to photopolymerize it at higher wavelengths. Theoretically, it seems doable, but I'm unsure about the chemistry~
16 January 2023 1,657 2 View
I assume that the antibody recognizes and attaches to the protein from the outer cell membrane. So, maybe there's no need for permeabilization? Bonus question: can we run sequential staining for...
24 November 2022 8,504 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View