I am using a neutralized collagen and upon cross linkage in the incubator, the gel becomes opaque. Is there a way to make it perfectly transparent?
I am trying to quantify Parylene C's hermetic encapsulation quality using EIS with a 2 electrode configuration (WE/WS and RE/CE). I am simply using two patterned gold layers on glass as my...
12 January 2021 7,399 14 View
I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.
01 December 2020 1,099 2 View
A simple definition might help to understand the diffraction pattern in an aperiodic nano-micro structured surface.
19 July 2018 3,865 6 View
01 June 2016 5,159 3 View
24 December 2015 6,303 4 View
24 November 2015 8,669 6 View
24 November 2015 8,487 5 View
Can tuning roughness leads to different stiffness of a material?
18 October 2015 3,286 8 View
As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether...
22 February 2013 5,130 2 View
While encapsulating ADMSCs in sodium alginate, providing a 3D environment for the cells, I come across different viability rates. Trypan blue shows a lower viability rate, as low as 50%. On the...
19 February 2013 6,200 5 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
24 February 2021 2,362 5 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View