I am interested in seeing the 3D image of the anterior chamber of the mouse eye. Due to the complexity of ethical permits, transporting the mice to the OCT scanner is challenging and will take a long time (😑). So, I was wondering if I could scan the fixed tissue instead. Are there any challenges in doing that?
In my opinion, I feel that would depend on the fixative method adopted. Also considering the size of the mouse's eye, fixing the specimen would result in some degree of tissue shrinkage which could cause disparity in your measurements.
Absolutely, you can definitely scan fixed tissue with OCT, and it's a pretty common method in research when live scanning isn't an option. Your idea to simplify things a bit is great! There are a few considerations, though:
So, while there might be a few more things to keep in mind, it's definitely feasible. Go ahead, give it a try and don't hesitate to ask if you have more questions. Best of luck with your imaging! 👍😊