I am using a sterilized GelMA to print structures using two-photon polymerization techniques. The printing procedure is carried out outside a sterilized (laminar flow hood, for example) environment, and I wondered how I could make the gels sterilized again after printing.
I would appreciate any insight or sharing of your experiences.
In general you can immerse the gel to the ethanol or propanol solution for several hours. Article Macroporous 3D printed structures for regenerative medicine ...
Journal: Bioprinting Title: Macroporous 3D printed structures for regenerative medicine applications. by Muhammad Moazzam, Ahmer Shehzad; Dana Sultanova; Fariza Mukasheva , Berillo D.A.; Dana Akilbekova 2022
Hi Hanie,
Depending on the photoinitiator you use, you can also try UV irradiation of the printed structure.
Alternatively, to bypass sterilization completely, you can try using sterile GelMA (like the GelMA from OkaSciences) in a biosafety cabinet if you have access to one, most bioprinters will fit inside these hoods.
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