I am using glutaraldehyde to fix cells on glass slides. However, in the next process I want to detach these cells off the slide.
Is there any detaching process defined for such situations? An anti-crosslinking procedure maybe?!
Simon Christopher Riley
Dear Hanie. Perhaps this depends on what you are trying to achieve? Can you run parallel samples - half you fix and view, the other half you detach in the normal way (scrape? trypsin? - it depends on what you are trying to achieve) for RNA? (trypsin or scrape) or protein? (trypsin will compromise surface proteins, so scrape). Or alternatively, can you detach and then fix? Good luck
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