By a 'higher differentiation capacity' I mean that it would be easier to differentiate them– especially without adding a pool of chemical factors.
I think cell lines might be tricky as they are somehow modified but stem cells (MSC, iPSC) might be more potent as they are there to differentiate.
Any insight is welcomed!
Dear Researcher
If you use scaffold that provide microenvironment for culture cells I think mesenchymal stem cells is better choice.
Thanks Arash Abdolmaleki , in fact that's what I'm thinking of. Im just looking for the best candidate.
iPSC will differentiate simply by changing the medium or letting them overgrow. You have to work to maintain their pluripotency. MSC will also differentiate somewhat over time, but generally it is a matter of changing the medium to include some inducers. Cell lines, by their nature, usually remain in a de-differentiated state unless you do something to them.
Hello Hanie Kavand
You should go for induced pluripotent stem cells (iPSCs). Induced pluripotent stem cells are somatic cells that have been reprogrammed to behave like an embryonic stem cell by artificially “turning on” expression of specific pluripotency genes. These cells are the most promising among those classified as pluripotent because of their high plasticity. They have the potential for self-renewal and can differentiate into any adult cell type. Many iPS cell lines share gene expression patterns and epigenetic traits of embryonic stem cells.
On the other hand, MSCs are a type of multipotent stem cells and cell lines as you mentioned maintain some degree of differentiation.
Best.
Thanks Marcus O. Muench . Right, so we will keep the cell line out. But lets imagine that we have a scaffold that is expected to mimic the physiological niche and we only want to use a normal media (not including growth factors) for differentiation. Which one of the 'stem cells' will be prone to differentiate more readily?
I know that with iPSs, they change the media in a time specific manner and use special media (and growth factors in each interval) to get differentiated cells. However, with MSCs its probably more easier; simply use a differentiation media throughout the experiment.
I believe iPSCs are 'wilder' and need to be controlled (=tamed) very closely thus maybe it is harder to differentiate them with biophysical cues (no special chemical involved)
Thanks Malcolm Nobre for the explanantion.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes