How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

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Alcian blue staining protocol for glutaraldehyde-fixed chondrocytes?

I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...

24 October 2023 7,385 0 View

Does anyone have experience extracting miRNA from serum?

Any suggestions for high quality miRNA extraction would be appreciated.

13 June 2023 5,958 3 View

How can I design a laparoscop in zemax?

Please help me. I don't know how can I design laparoscope with zemax.

01 April 2023 3,150 0 View

How to inject beads/organoids into microfluidic devices with high loading yields?

I use the typical syringe pumps to load organoids (200-300 um in diameter) into my device; however, I don't see them in the chip! I swapped them with beads (similar size) instead and observed that...

26 February 2023 944 2 View

Fluorescence vs. autofluorescence: What is the correct terminology to use when talking about cells, proteins, or any other material?

It's quite confusing to distinguish between the definitions. To me, autofluorescence fits all. But what is the correct terminology to use when talking about biological cells, proteins (such as...

14 February 2023 969 5 View

Should the cell permeabilization step for immunostaining of tissues (whole mount) be more intense?

I am trying to immunostain whole (micro)tissue samples, and I think there is an antibody penetration issue. Would longer permeabilization procedures or harsher permeabilization agents help with...

14 February 2023 5,892 4 View

Protocol for picrosirius red staining chondrocyte culture?

Hi, I fixed chondrocytes in 12-well plate with glutaraldehyde. I want to stain the cells by direct red. Do you have any protocol? Thank you.

04 February 2023 6,914 0 View

Is delta Ct=10 reasonable for TNF-a in OA cartilage?

Hi, I performed RT-PCR directly on the osteoarthritic cartilages obtained from 2 patients. In both experiments, Ct for TNF-a was found to be around 36, which is unexpected considering the...

31 January 2023 7,269 0 View

Can we add a second photo-initiator (near IR) to SU-8?

I was wondering if it is possible to add a second photoinitiator to SU-8 to photopolymerize it at higher wavelengths. Theoretically, it seems doable, but I'm unsure about the chemistry~

16 January 2023 1,657 2 View

Is cell permeabilization necessary for immunocytochemistry staining of transmembrane proteins?

I assume that the antibody recognizes and attaches to the protein from the outer cell membrane. So, maybe there's no need for permeabilization? Bonus question: can we run sequential staining for...

24 November 2022 8,504 2 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

How to clean the CAD detector?

The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...

01 August 2024 7,805 0 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

How to find a reaction is reversible or irreversible in CV? How to know the number of electrons transfer?

My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...

30 July 2024 4,671 3 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

While doing Chronoamperometry, when the light is turned off, why the current is not decreasing sharply?

Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.

29 July 2024 4,538 3 View

Which filtration method to go for run off water from dirty solar panels to be used again?

We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...

28 July 2024 5,778 2 View

Why semicircle of nyquist plot is not complete?

While I am doing HER, I noticed that nyquist plots of Electrochemical Impedance Spectroscopy (EIS) is not complete. Will it affect for the Linear Sweep Voltametry (LSV) data? However, I can found...

26 July 2024 7,542 4 View