As trypan blue fails to calculate the accurate viability rate of my [cell+alginate 1.2%] suspension, I was thinking about using dual florescent dye (PI & AO). However, I am concerned about whether low viscous solutions are compatible with flow cytometry or not?
Why don't you separate your cells from the alginate solution? You can assess the flow cytometry test immediately after the separation. You can find something similar in one of my papers: http://www.biomedcentral.com/1472-6750/12/35.
Good luck.
Thank you so much Bianca for taking interest in my questions!
In the process of cell extraction from the alginate beads, using sodium citrate, the cells will go under tensions and stress and hence the true viability rate will be affected. However, if we could have assets the viability right after suspension in the alginate solution, we could have understood the real time effect of the solution on the suspended cells. This questioned popped in my mind because I was experiencing a dramatical decrease in my viability. So, i kept wondering where the problem might be.
- Badges
- Science method