We are currently using Ketamine and Xylazine to anesthetize the mice for imaging purposes. However, upon injection, we see an increase in the blood glucose levels, which in turn, prevents us from inducing glucose stimulation at our desired time point. Is there any anesthetic material that doesn't interfere with pancreatic beta cells or elevate blood glucose levels?
Any insights will be very helpful.
Unfortunately, there is no such anesthesia. All form of anesthesia, both liquid and gaseous, increase the level of stress hormones and hence, the glucose stimulation. If you are also performing surgery on the animals, the procedure itself will elevate the blood glucose level. By the way, this might be a question to rise to an anesthesiologist.
Thanks, Kajanthan Piraisoody . It appears like Fentanyl used to be the perfect anesthetic for such experiments, but the drug is now banned.
I also found Pentobarbital, but again, we don't have permission to use it.
Ketamine is sympathomimetic drugs, result in increased blood glucose. Usually
mixture of Ketamine and propofol in ratio of 1:1 limit the increase blood glucose level.
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