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Questions related from Annadurai Shanmuganathan
We need to collect the blood samples 28 times in the period of one week. The required sample volume is 300 microlitre on each collection. We tried to collect the sample from tail vein. However, we...
03 August 2017 991 3 View
I need to feed the rat with powder high fat diet. We used to mix the powder high fat diet with coconut oil and make the powder diet into small balls. But, the rats are not eating these balls...
17 November 2016 6,544 5 View
There are four groups in our study and we measured the study parameters in two different time period in each group. So, there are two data for each group, for instance food intake from 01-30 days...
04 November 2016 6,379 1 View
I do TBARS assay by following the protocol of Ohkawa et al, 1979 (Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction). I need a clarification on the working protocol this...
12 August 2016 1,581 4 View
I need to prepare diet induced hyperlipidemic rat model. In literature, numerous protocol of high fat diet preparation have been given. While feeding with wet diet, rats are not eating properly...
01 March 2016 2,453 8 View
As you all know, the concentration of various biological substances are being quantified through calorimetric assays. Most of the protocol, the given formula to calculate the concentration of...
27 May 2015 1,068 4 View
We are going to screen the some herbal drugs for its hypolipidemic effect. In this experiment, zebrafish (8 or 9 day post fertilization) must be stained with Oil Red O staining to visualize the...
22 March 2015 3,231 2 View
We have planned to buy a microplate reader (absorbance) for our laboratory. The available funding is Rs.4,50,000 (7000 dollars). While enquiry, we came to know that filter based readers are...
06 March 2015 3,660 4 View
Usually, when we are preparing the solution by mixing the solvent and solute, I understand that it has been dissolved or not, only by inspection. But, I want to know if there is any parameter by...
23 February 2015 2,459 3 View
I need to execute the experiment on Triton WR 1339 induced hyperlipidemic rat model. The dose of Triton is 200mg/kg of body weight. I need some tips concerning this: 1. It is observed that many...
15 February 2015 9,128 2 View
The test 'lipid profile' is being prescribed by physicians and also performed by researchers concerning dyslipidemia. Mostly, they are measuring the total cholesterol, triglycerides, HDL-C and...
14 February 2015 7,167 16 View
The level of malonaldehyde (MDA) is being measured from both tissue and serum samples concerning lipid peroxidation. In literature, the unit of MDA level in serum is mentioned as micro mole /...
26 January 2015 6,983 6 View
In order to screen the hypolipidemic drugs, Triton induced hyperlipidemic animal model is being used. In most of the papers, Triton WR 1339 (tyloxapal) was used to induce hyperlipidemia. However,...
25 January 2015 2,251 3 View
Many companies providing single reagent solution for enzymatic reactions, by which we can quantify cholesterol, triglycerides, and HDL-C. Can I use the these human compatible reagents with rat or...
25 January 2015 885 9 View
We need to measure total lipids content in a serum sample by using colorimetric assay and pertaining to this, which chemical is used as standard. Thanks many for your replies.
11 January 2015 2,876 3 View
Please suggest me about standard protocol to measure the plasma free fatty acid level by spectrophotometry. Thanking you in advance.
01 January 2015 1,584 5 View
We need to test our herbal drug with diet induced hyperlipidemic animal model (Sprague Dawley rats) and we have gone through literature to know about high fat diet. There are many protocols...
26 December 2014 8,983 2 View
I have read the protocol of the protein estimation by Lowry's method from many sources such as theses, published papers, etc. The entire protocol is similar except the nm in which the colour to be...
01 December 2014 1,855 3 View
For histopathological studies, It is necessary to make whole animal perfusion on the laboratory experimental animals, i.e. rat or mice. What are the parameters can be considered to confirm that,...
01 November 2014 7,264 9 View
In India, is it necessary to obtain the ethical clearance so as to use the Zebrafish for research purposes. In CPCSEA guidelines, nothing was mentioned about Zebrafish. I need an advice in this...
24 October 2014 2,697 13 View
I need to measure the corticosterone level in rat blood by any calorimetric assay. If any one has experience with this parameter, please recommend a protocol, which is ideal to measure the blood...
16 October 2014 2,632 4 View
We are going to buy a real time PCR instrument for our laboratory and we received a quotation from BioRad, Applied Biosystem and Roche companies. The price of all three makes is feasible with our...
10 October 2014 3,746 15 View
I understand that there are several methods available to prepare plant extract namely maceration, decoction, infusion, Soxhlet Extraction, Microwave assisted extraction (MAE), supercritical fluid...
02 October 2014 8,048 8 View
It well known that, the protocol of Triton WR 1339 induced hyperlipidemic model is the standard method to screen the herbals for hypolipidemic action. I have read several papers in which there...
18 September 2014 7,375 2 View
I need to measure the total cholesterol from serum and liver tissue samples. We don't have enough funds to procure kits. While searching by literature, i observed several colorimetric assays to...
12 September 2014 6,150 1 View
In order to perform a colorimetric assay (by spectrophotometer), generally two types of protocol were described in literature, i.e. standard assay vs micro plate assay. In common perspective, i do...
08 September 2014 3,512 3 View
It is apparent that, various parts of plant material are being used to treat many human illnesses from ancient time to present. If we screen the herbals for their biological action in literature,...
04 September 2014 6,548 12 View
As we know that, direct measurement of LDL-C (low density lipoproteins) requires sophisticated equipments. Hence, LDL concentration is being calculated through Friedewald equation in many...
30 August 2014 786 15 View
This may be a simple question, however, I face this problem every day. As you aware, there is a need to analyze the weight of the animal during study period. While weighing in an electronic scale,...
23 August 2014 5,520 19 View
As we know that microarray and qPCR are both being employed to study the gene expression and arbitrarily, 2 fold change is considered to declare the genes as deferentially expressed. What is the...
14 August 2014 8,197 6 View
In human, we can get a capillary blood by finger pricking method. I need to know about the methods in rat to collect the capillary blood sample.
22 June 2014 492 3 View
Is there any specific protocol to quantify vitamin C concentration in skeletal muscle tissue samples? While scanning the literature I came to understand the HPLC assay is the standard method to...
21 May 2014 3,575 3 View
The drug concentration to be given to the rat is 400mg/kg. If I prepare the 8% stock drug solution, 4gm of the extract is dissolved in 50ml of the solvent. Theoretically, if I take 1 ml from the...
05 April 2014 3,848 3 View
In our experiment, we have got to quantify the GLUT 1 Protein, expressed in RBCs of two different groups (Human Blood Sample). While considering the facility in our laboratory, there are two...
01 February 2014 1,369 10 View
Usually, blood glucose concentration is measured in plasma or serum. I need to measure the glucose concentration in red blood cells (intracellular RBC glucose concentration) in normal and...
16 November 2013 9,019 9 View
I am very sorry to encroach your time by asking very basic questions. 1. I understood that a gene is a nucleotide bases in the template strand (non-coding strand or non-sense strand). Am I...
07 September 2013 9,720 2 View
I want to measure the expression of a target between two conditions such as cancer vs normal. To achieve this, which method is ideal, i.e. whether absolute quantification or relative quantification?
12 August 2013 4,507 2 View
Wish to investigate to confirm that whether a protein of interest is expressed or not expressed in the particular cell types. The screening of this particular protein molecule is only from normal...
01 January 1970 4,945 3 View
As known that Qualitative real time RT-PCR is being employed to diagnose the COVID-19 disease and it is perceived that there is a possibility of false-negative results in COVID-19 testing, and low...
01 January 1970 3,316 3 View
