19 Questions 9 Answers 0 Followers
Questions related from Annadurai Shanmuganathan
I need to feed the rat with powder high fat diet. We used to mix the powder high fat diet with coconut oil and make the powder diet into small balls. But, the rats are not eating these balls...
17 November 2016 6,427 5 View
There are four groups in our study and we measured the study parameters in two different time period in each group. So, there are two data for each group, for instance food intake from 01-30 days...
04 November 2016 6,305 1 View
I need to prepare diet induced hyperlipidemic rat model. In literature, numerous protocol of high fat diet preparation have been given. While feeding with wet diet, rats are not eating properly...
01 March 2016 2,388 8 View
As you all know, the concentration of various biological substances are being quantified through calorimetric assays. Most of the protocol, the given formula to calculate the concentration of...
27 May 2015 997 4 View
We are going to screen the some herbal drugs for its hypolipidemic effect. In this experiment, zebrafish (8 or 9 day post fertilization) must be stained with Oil Red O staining to visualize the...
22 March 2015 3,163 2 View
Usually, when we are preparing the solution by mixing the solvent and solute, I understand that it has been dissolved or not, only by inspection. But, I want to know if there is any parameter by...
23 February 2015 2,373 3 View
In order to screen the hypolipidemic drugs, Triton induced hyperlipidemic animal model is being used. In most of the papers, Triton WR 1339 (tyloxapal) was used to induce hyperlipidemia. However,...
25 January 2015 2,182 3 View
We need to test our herbal drug with diet induced hyperlipidemic animal model (Sprague Dawley rats) and we have gone through literature to know about high fat diet. There are many protocols...
26 December 2014 8,918 2 View
We are going to buy a real time PCR instrument for our laboratory and we received a quotation from BioRad, Applied Biosystem and Roche companies. The price of all three makes is feasible with our...
10 October 2014 3,637 15 View
I understand that there are several methods available to prepare plant extract namely maceration, decoction, infusion, Soxhlet Extraction, Microwave assisted extraction (MAE), supercritical fluid...
02 October 2014 7,960 8 View
In order to perform a colorimetric assay (by spectrophotometer), generally two types of protocol were described in literature, i.e. standard assay vs micro plate assay. In common perspective, i do...
08 September 2014 3,424 3 View
As we know that microarray and qPCR are both being employed to study the gene expression and arbitrarily, 2 fold change is considered to declare the genes as deferentially expressed. What is the...
14 August 2014 8,141 6 View
In human, we can get a capillary blood by finger pricking method. I need to know about the methods in rat to collect the capillary blood sample.
22 June 2014 440 3 View
Is there any specific protocol to quantify vitamin C concentration in skeletal muscle tissue samples? While scanning the literature I came to understand the HPLC assay is the standard method to...
21 May 2014 3,532 3 View
The drug concentration to be given to the rat is 400mg/kg. If I prepare the 8% stock drug solution, 4gm of the extract is dissolved in 50ml of the solvent. Theoretically, if I take 1 ml from the...
05 April 2014 3,778 3 View
In our experiment, we have got to quantify the GLUT 1 Protein, expressed in RBCs of two different groups (Human Blood Sample). While considering the facility in our laboratory, there are two...
01 February 2014 1,306 10 View
I am very sorry to encroach your time by asking very basic questions. 1. I understood that a gene is a nucleotide bases in the template strand (non-coding strand or non-sense strand). Am I...
07 September 2013 9,655 2 View
I want to measure the expression of a target between two conditions such as cancer vs normal. To achieve this, which method is ideal, i.e. whether absolute quantification or relative quantification?
12 August 2013 4,456 2 View
Wish to investigate to confirm that whether a protein of interest is expressed or not expressed in the particular cell types. The screening of this particular protein molecule is only from normal...
01 January 1970 4,875 3 View
