We need to collect the blood samples 28 times in the period of one week. The required sample volume is 300 microlitre on each collection. We tried to collect the sample from tail vein. However, we could not able to collect consistently from tail vein, i.e. sometime it is feasible, sometime not. Hence, we are seeking a suitable blood collection method for this case.
Thanking You in advance for your kind help in this regard.
My late colleague Gerard Beuving tackled a similar problem in laying hens with a permanent canula in the wing vein, which remained active for about 10 days: see this link
https://scholar.google.com/scholar_lookup?title=Effect%20of%20stressing%20factors%20on%20corticosteronelevels%20inthe%20plasma%20of%20laying%20hens&author=G.%20Beuving&author=GMA.%20Vonder&journal=Gen.Comp.Endoerinoi.&volume=35&pages=153-159&publication_year=1978
or these references
Beuving, G. and Vonder, G.M.A. 1978. Effect of stressing factors on corticosterone levels inthe plasma of laying hens. Gen.Comp.Endocrinol. 35, 153–159.
Beuving, G. and Vonder, G.M.A. 1981. The influence of ovulation and ovi-position on corticosterone levels in the plasma of laying hens. Gen. Comp.Endocrinol. 44, 382–388.
https://www.neoteryx.com/microsampling-blog/the-top-methods-of-blood-collection-in-rodents
https://www.ncbi.nlm.nih.gov/pubmed/4351485
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