I need to measure the total cholesterol from serum and liver tissue samples. We don't have enough funds to procure kits. While searching by literature, i observed several colorimetric assays to measure total cholesterol, i.e.
1. Direct manual determination of serum total cholesterol with a single stable reagent. Wybenga DR, Pileggi VJ, Dirstine PH, Di Giorgio J.
2. Colorimetry of serum cholesterol with use of ferric acetate/uranyl acetate and ferrous sulfate/sulfuric acid reagents.Jung DH, Biggs HG, Moorehead WR.
Please suggest me about the best colorimetric assay to measure total cholesterol. Thanking you in advance for sharing your knowledge.
Dear Sir,
I am greatly indebted for your reply. I will be very thankful, if you send a protocol, what you already used in your laboratory.
Email ID: [email protected]
Thank You.