We are going to screen the some herbal drugs for its hypolipidemic effect. In this experiment, zebrafish (8 or 9 day post fertilization) must be stained with Oil Red O staining to visualize the lipids. If any one has a working protocol of Oil Red O staining pertaining to zebrafish, please share with us.
Thanking you in advance for sharing your knowledge.
Hi:
Oil red O is not the only drug for detecting lipid, and its sensitivity is not so good for small lipid droplets. I suggest you can try BODIPY® FL-C12 (https://www.lifetechnologies.com/order/catalog/product/D3822), it has different fluorescent colors and is highly sensitive for detecting lipid droplets. Usually, it can be used to monitor lipogenesis or lipolysis in fixed cells or realtime detection. I try Oil red and BODIPY® FL-C12 for my experiment, BODIPY® FL-C12 is very easy to handle and higher sensitivity than Oil red, for the protocol please the attachment.
You can use Oil Red O for detection of lipid droplets. For example, I have used Oil Red O staining in Zebrafish larvae of 7dpf which has already been in the low concentration PTU treated one (so that melanin formation can be inhibited) and observed the liver under the microscope. The fatty liver which occurs sometimes due to a hepatotoxic drug can be visualized with Oil Red O. But I don't think you can measure the lipids in the blood stream. I don't think you can inject it into the circulation as well because Oil Red O is not easily soluble in water and you need to add glycerol.
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