PFA6a - mRFP - kanMX6 - plasmid whether integrative or replicative plasmid??

pFA6a - mRFP - kanMX6 - plasmid whether integrative or replicative plasmid?? How to confirm, whether this RFP (Red fluorescent protein) plasmid integrative or replicative?

11 March 2018 615 1 View

I need a help to develop the matlab coding for structural health monitoring.please kindly help me

Dear friends, i am doing the project in structural health monitoring. i need a help to develop and implement the coding matlab. please kindly help me. looking positive information

02 August 2017 5,377 9 View

Ideal way to administer the powder diet into rat?

I need to feed the rat with powder high fat diet. We used to mix the powder high fat diet with coconut oil and make the powder diet into small balls. But, the rats are not eating these balls...

16 November 2016 6,341 5 View

How to compare the groups in which each group consists the data for two different duration?

03 November 2016 6,237 1 View

Is it possible to calculate power for packet delivery rate?

I have a doubt to calculate the packet delivery rate in sensornetwork? is there any general formulae to calculate the power for packet delivery rate? please kindly provide the solution. thank you

17 August 2015 5,763 8 View

How do you confirm the solubility apart from inspection?

22 February 2015 2,299 3 View

Which triton (x 100 or wr1339) might be used to induce hyperlipidemia?

In order to screen the hypolipidemic drugs, Triton induced hyperlipidemic animal model is being used. In most of the papers, Triton WR 1339 (tyloxapal) was used to induce hyperlipidemia. However,...

24 January 2015 2,111 3 View

Does any one experience with ideal diet induced hyperlipidemic animal model?

We need to test our herbal drug with diet induced hyperlipidemic animal model (Sprague Dawley rats) and we have gone through literature to know about high fat diet. There are many protocols...

25 December 2014 8,858 2 View

Which is the best real time PCR detection system among BioRad, Applied Biosystem and Roche?

We are going to buy a real time PCR instrument for our laboratory and we received a quotation from BioRad, Applied Biosystem and Roche companies. The price of all three makes is feasible with our...

09 October 2014 3,571 15 View

Which is the best method for preparing plant extracts with polar, non-polar and mid-polar solvents?

I understand that there are several methods available to prepare plant extract namely maceration, decoction, infusion, Soxhlet Extraction, Microwave assisted extraction (MAE), supercritical fluid...

01 October 2014 7,894 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View