Testosterone or DHT deficient mice or conditional knockout available?

Hi there I've a congenic strain of mice that exhibits a phenotype dependent on testis (lost following orchiectomy) and was wondering if i can assess the contribution of Testosterone or DHT using a...

09 October 2018 2,379 2 View

Looking for some help converting genomic coordinates to genes?

Hi there I've a total of 8000 list of genomic coordinates from mouse that I want to convert to gene names, is there a software out there that can do it quickly rather than I doing them manually...

06 July 2016 3,880 6 View

How to collate sequence data from different databases in excel ?

Hi there I am analyzing SNPs from two different datasets that once collated will provide a complete picture of haplotype blocks. The issue I am facing here is there are SNPs that are found in both...

06 July 2016 8,081 1 View

Mouse SNP query: duplication of strain data?

06 July 2016 3,973 1 View

How much Retro-orbital blood can we safely withdraw from mice?

Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...

08 September 2015 7,685 3 View

What is the dihydrotestosterone dosage in mice for oral administration?

Hi there, i am looking for the appropriate dosage of DHT in C57BL/6 mice that can be given orally to compensate for gdx. I haven't found an appropriate study that has used it. If any investigator...

05 June 2015 8,293 5 View

Does anyone know about silastic tubing for implants in mice?

Hi there, i am looking for some guidelines to using silastic tubing for implanting hormones in mice. I need to provide 50ug of drug released every day. Has anyone worked with them and would be...

05 June 2015 2,360 5 View

Ubiquitin turnover controlled by?

03 April 2015 449 0 View

How can I get around the fact that the H1R GPCR multimer upon lysate preparation gets denatured and aggregates on the SDS PAGE gel?

I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my...

02 March 2015 4,230 6 View

Has anyone come across S-myc tag rather than the conventional c-myc protein tag? Can we detect this protein tag using anti-c-myc antibodies or not?

Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...

01 February 2015 10,018 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View