Testosterone or DHT deficient mice or conditional knockout available?

Hi there I've a congenic strain of mice that exhibits a phenotype dependent on testis (lost following orchiectomy) and was wondering if i can assess the contribution of Testosterone or DHT using a...

09 October 2018 2,379 2 View

Looking for some help converting genomic coordinates to genes?

Hi there I've a total of 8000 list of genomic coordinates from mouse that I want to convert to gene names, is there a software out there that can do it quickly rather than I doing them manually...

06 July 2016 3,880 6 View

How to collate sequence data from different databases in excel ?

Hi there I am analyzing SNPs from two different datasets that once collated will provide a complete picture of haplotype blocks. The issue I am facing here is there are SNPs that are found in both...

06 July 2016 8,081 1 View

Mouse SNP query: duplication of strain data?

06 July 2016 3,973 1 View

How much Retro-orbital blood can we safely withdraw from mice?

Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...

08 September 2015 7,685 3 View

What is the dihydrotestosterone dosage in mice for oral administration?

Hi there, i am looking for the appropriate dosage of DHT in C57BL/6 mice that can be given orally to compensate for gdx. I haven't found an appropriate study that has used it. If any investigator...

05 June 2015 8,293 5 View

Does anyone know about silastic tubing for implants in mice?

Hi there, i am looking for some guidelines to using silastic tubing for implanting hormones in mice. I need to provide 50ug of drug released every day. Has anyone worked with them and would be...

05 June 2015 2,360 5 View

Ubiquitin turnover controlled by?

03 April 2015 449 0 View

How can I get around the fact that the H1R GPCR multimer upon lysate preparation gets denatured and aggregates on the SDS PAGE gel?

I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my...

02 March 2015 4,230 6 View

Has anyone come across S-myc tag rather than the conventional c-myc protein tag? Can we detect this protein tag using anti-c-myc antibodies or not?

Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...

01 February 2015 10,018 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View