How is the rate constant enhanced in enzyme kinetics?

05 May 2014 1 4K Report

There are many ways through which enzymes increase their rate constants and one of them is called as encounter complex. I am looking for an explanation of this process. Any help or figure would be highly appreciated.

René Renato Balandrán-Quintana

See this paper: Marcellus Ubbink. The courtship of proteins: Understanding the encounter complex. FEBS Letters 583 (2009) 1060–1066.

Best

Badges
Science topic

More Abbas Raza's questions See All

Testosterone or DHT deficient mice or conditional knockout available?

Hi there I've a congenic strain of mice that exhibits a phenotype dependent on testis (lost following orchiectomy) and was wondering if i can assess the contribution of Testosterone or DHT using a...

09 October 2018 2,551 2 View

Looking for some help converting genomic coordinates to genes?

Hi there I've a total of 8000 list of genomic coordinates from mouse that I want to convert to gene names, is there a software out there that can do it quickly rather than I doing them manually...

06 July 2016 4,013 6 View

How to collate sequence data from different databases in excel ?

Hi there I am analyzing SNPs from two different datasets that once collated will provide a complete picture of haplotype blocks. The issue I am facing here is there are SNPs that are found in both...

06 July 2016 8,245 1 View

Mouse SNP query: duplication of strain data?

I am looking at SNPs between different strains of mice for a particular interval. When I try to use the MGI for SNP analysis http://www.informatics.jax.org/ I find strains that have multiple data...

06 July 2016 4,133 1 View

How much Retro-orbital blood can we safely withdraw from mice?

Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...

08 September 2015 7,838 3 View

What is the dihydrotestosterone dosage in mice for oral administration?

Hi there, i am looking for the appropriate dosage of DHT in C57BL/6 mice that can be given orally to compensate for gdx. I haven't found an appropriate study that has used it. If any investigator...

05 June 2015 8,424 5 View

Does anyone know about silastic tubing for implants in mice?

Hi there, i am looking for some guidelines to using silastic tubing for implanting hormones in mice. I need to provide 50ug of drug released every day. Has anyone worked with them and would be...

05 June 2015 2,511 5 View

Ubiquitin turnover controlled by?

I am looking for an explanation regarding ubiquitin-proteosome-system of protein degradation: 1. How is ubiquitin itself turned over? 2. Why proteosome don't break peptide bonds in ubiquitn when...

03 April 2015 591 0 View

How can I get around the fact that the H1R GPCR multimer upon lysate preparation gets denatured and aggregates on the SDS PAGE gel?

I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my...

02 March 2015 4,363 6 View

Has anyone come across S-myc tag rather than the conventional c-myc protein tag? Can we detect this protein tag using anti-c-myc antibodies or not?

Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...

01 February 2015 10,129 6 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View