Looking for some help converting genomic coordinates to genes?

Hi there I've a total of 8000 list of genomic coordinates from mouse that I want to convert to gene names, is there a software out there that can do it quickly rather than I doing them manually...

06 July 2016 3,880 6 View

How to collate sequence data from different databases in excel ?

Hi there I am analyzing SNPs from two different datasets that once collated will provide a complete picture of haplotype blocks. The issue I am facing here is there are SNPs that are found in both...

06 July 2016 8,081 1 View

Mouse SNP query: duplication of strain data?

06 July 2016 3,973 1 View

How much Retro-orbital blood can we safely withdraw from mice?

Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...

08 September 2015 7,685 3 View

What is the dihydrotestosterone dosage in mice for oral administration?

Hi there, i am looking for the appropriate dosage of DHT in C57BL/6 mice that can be given orally to compensate for gdx. I haven't found an appropriate study that has used it. If any investigator...

05 June 2015 8,293 5 View

Does anyone know about silastic tubing for implants in mice?

Hi there, i am looking for some guidelines to using silastic tubing for implanting hormones in mice. I need to provide 50ug of drug released every day. Has anyone worked with them and would be...

05 June 2015 2,360 5 View

Ubiquitin turnover controlled by?

03 April 2015 449 0 View

How can I get around the fact that the H1R GPCR multimer upon lysate preparation gets denatured and aggregates on the SDS PAGE gel?

I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my...

02 March 2015 4,230 6 View

Has anyone come across S-myc tag rather than the conventional c-myc protein tag? Can we detect this protein tag using anti-c-myc antibodies or not?

Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...

01 February 2015 10,018 6 View

Has anyone found double positive TCRb and TCRgd immune cells?

01 February 2015 8,152 7 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Hi, if we isolate and grow mouse primary myoblast or cardiomyocytes, can we see the dystrophin by immuno or we need to differentiate first?

We want to measure dystrophin restoration after a treatment in DMD-KO mouse model by immuno or western.

01 February 2021 4,907 2 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View