Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no homology as far as I can see in the two tags. If anyone has used this tag or can guide me about it like can we detect the protein using anti-c-myc antibodies or not?
Hi Abbas,
I have come across the S tag (not an S-myc tag), a newer epitope tag that is unrelated to myc. I'm fairly certain you will not be able to detect it with an antibody against c-myc.
Hi Abbas,
I have come across the S tag (not an S-myc tag), a newer epitope tag that is unrelated to myc. I'm fairly certain you will not be able to detect it with an antibody against c-myc.
Hi Abbas, would you mind posting the peptide sequence of your S-myc Tag?
This is the S tag epitope that I mentioned: KETAAAKFERQHMDS
which is unrelated to c-myc, at least in primary structure terms: EQKLISEEDL
Does that match what you have, Abbas?
thanks Mark, i have the problem sorted. the person who had provided me the construct had included a signal sequence and labeled it S-myc.
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