I am working with histamine receptor 1, a G protein couple receptor (GPCR), and when i prep my lysate for SDS page gel run I heat them up at 95C/3 min. I am getting protein aggregation in my western bands. I tried 65C/10 min and still the pattern isn't changed. Has anyone worked with H1R and used any strategy to get around this problem.
I am not sure if its the glycosylated H1R that is causing this multimer pattern of GPCR