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Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View

How do we assess information security in a public organisation?

Dear all, I am wondering if anyone can point me to studies discussing non-technical measurement tools to assess information security in a public organisation. I am planning to explore the...

23 November 2020 3,497 6 View

Any suggestions for the specs (specifications) of a laptop/desktop computer that can be used for Molecular Docking Tools/Software?

I'm a researcher that will be using USCF Chimera, Autodock, and Autodock Vina tools. I've been using an Intel i5 8th Gen processor (i5-8250U) lenovo E480 thinkpad with 8GB of RAM, and I'm planning...

18 November 2020 3,578 3 View

How do you design heterogeneity spacers for primers in amplicon metagenomic studies?

Adding heterogeneity spacers to primers used for Illumina based amplicon studies has proven to improve read quality by increasing bp diversity on the flow cell during sequencing. I have seen two...

01 November 2020 9,071 3 View

How do I collect reviews in several languages from TripAdvisor?

I have been trying to extract data from TripAdvisor for my Master thesis. However, I can get only the english reviews. The reviews in other languages I can not extract. If someone could help,...

10 October 2020 6,382 2 View

How useful would a Predictive CAD systems be?

Hi, I wondered if you could spare a few minutes to watch this short video of a prototype Predictive CAD system (created by our research project) and answer some questions afterwards? We are...

06 October 2020 1,626 3 View

In linear regression, can I reverse-engineer r-squared if I know sample size, beta, standard error, and the p-value?

I'm doing a literature review, and most of the data I need come from linear regression analyses. There's a paper that has solid data, but it doesn't report r-squared, which is the metric I need....

15 September 2020 208 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View