Does anyone can help me to download this paper?

The title's paper is: Development and validation of a rapid and reliable method for TPMT genotyping using real-time PCR. Author: Lorenz et al.2012. Clin Lab. 58(9-10):959-71 Thanks everyone!

03 April 2018 907 1 View

Does anyone can help me to download this chapter's booK?

Studies in Logic and the Foundations of Mathematics Volume 25, 1959, Pages 31-51 Chapter 2 Aristotle's Theory of Science

03 April 2018 832 2 View

What is the difference between the prefix "let" (for example, in let-7) and "miR" (for example, in miR-100) in microRNAs nomenclature?

What does the prefix "let" (for example, in let-7)in microRNAs nomenclature means?

02 March 2018 6,638 0 View

In which acid I can dissolve medium molecular weight chitosan?

Which is better hydrochloric acid or acetic acid?why?

09 October 2015 552 3 View

How can I prepare sodium acetate (0.1M)/ acetic acid (0.1M) buffer pH 5.4?

How much volume I have to add of acetic acid and grams of sodium acetate?

09 October 2015 9,855 1 View

How much of DNA and Ethidium bromide I have to add in a tube if I wanna obtain a ratio of EtBr/DNA phosphate of 1:20?

08 September 2015 7,246 0 View

Which buffers can I use to ensure an acidic medium?( pH between 1-4)?

I'm making experiments with chitosan particles to capture DNA. I have read that in an acidic medium chitosan is in it's protonate state so the interaction between the DNA and the chitosan it's...

08 September 2015 7,920 3 View

Does anyone know the mechanism behind this result?

this is a reaction with citrate modified gold nanoparticles,thiolated probes and genomic DNA. First, i'm not sure for what reason you use thiolated probes, and second which is the mechanism behind...

04 May 2015 4,015 3 View

Which is the mechanism behind this reaction between thiolated probe, genomic DNA and gold nanoparticle?

I don't understand which is the mechanism behind the visual reaction? I mean how the thiolated probe, the gold nanoparticle and the genomic DNA interact so that you see a color change?

04 May 2015 1,536 4 View

What is the difference between using water and acetic acid when making chitosan particles?

with which group of the chitosan reacts the acetic acid?

03 April 2015 8,122 14 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View