Does anyone can help me to download this paper?

The title's paper is: Development and validation of a rapid and reliable method for TPMT genotyping using real-time PCR. Author: Lorenz et al.2012. Clin Lab. 58(9-10):959-71 Thanks everyone!

03 April 2018 907 1 View

Does anyone can help me to download this chapter's booK?

Studies in Logic and the Foundations of Mathematics Volume 25, 1959, Pages 31-51 Chapter 2 Aristotle's Theory of Science

03 April 2018 832 2 View

What is the difference between the prefix "let" (for example, in let-7) and "miR" (for example, in miR-100) in microRNAs nomenclature?

What does the prefix "let" (for example, in let-7)in microRNAs nomenclature means?

02 March 2018 6,638 0 View

Why the polyanionic nature of DNA facilitates the ionization of the glucosamines units of chitosan?

02 March 2016 5,474 0 View

In which acid I can dissolve medium molecular weight chitosan?

Which is better hydrochloric acid or acetic acid?why?

09 October 2015 552 3 View

How can I prepare sodium acetate (0.1M)/ acetic acid (0.1M) buffer pH 5.4?

How much volume I have to add of acetic acid and grams of sodium acetate?

09 October 2015 9,855 1 View

Which buffers can I use to ensure an acidic medium?( pH between 1-4)?

I'm making experiments with chitosan particles to capture DNA. I have read that in an acidic medium chitosan is in it's protonate state so the interaction between the DNA and the chitosan it's...

08 September 2015 7,920 3 View

Does anyone know the mechanism behind this result?

this is a reaction with citrate modified gold nanoparticles,thiolated probes and genomic DNA. First, i'm not sure for what reason you use thiolated probes, and second which is the mechanism behind...

04 May 2015 4,015 3 View

Which is the mechanism behind this reaction between thiolated probe, genomic DNA and gold nanoparticle?

I don't understand which is the mechanism behind the visual reaction? I mean how the thiolated probe, the gold nanoparticle and the genomic DNA interact so that you see a color change?

04 May 2015 1,536 4 View

What is the difference between using water and acetic acid when making chitosan particles?

with which group of the chitosan reacts the acetic acid?

03 April 2015 8,122 14 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View