Does anyone can help me to download this paper?

The title's paper is: Development and validation of a rapid and reliable method for TPMT genotyping using real-time PCR. Author: Lorenz et al.2012. Clin Lab. 58(9-10):959-71 Thanks everyone!

03 April 2018 907 1 View

Does anyone can help me to download this chapter's booK?

Studies in Logic and the Foundations of Mathematics Volume 25, 1959, Pages 31-51 Chapter 2 Aristotle's Theory of Science

03 April 2018 832 2 View

Why the polyanionic nature of DNA facilitates the ionization of the glucosamines units of chitosan?

02 March 2016 5,474 0 View

In which acid I can dissolve medium molecular weight chitosan?

Which is better hydrochloric acid or acetic acid?why?

09 October 2015 552 3 View

How can I prepare sodium acetate (0.1M)/ acetic acid (0.1M) buffer pH 5.4?

How much volume I have to add of acetic acid and grams of sodium acetate?

09 October 2015 9,855 1 View

How much of DNA and Ethidium bromide I have to add in a tube if I wanna obtain a ratio of EtBr/DNA phosphate of 1:20?

08 September 2015 7,246 0 View

Which buffers can I use to ensure an acidic medium?( pH between 1-4)?

I'm making experiments with chitosan particles to capture DNA. I have read that in an acidic medium chitosan is in it's protonate state so the interaction between the DNA and the chitosan it's...

08 September 2015 7,920 3 View

Does anyone know the mechanism behind this result?

this is a reaction with citrate modified gold nanoparticles,thiolated probes and genomic DNA. First, i'm not sure for what reason you use thiolated probes, and second which is the mechanism behind...

04 May 2015 4,015 3 View

Which is the mechanism behind this reaction between thiolated probe, genomic DNA and gold nanoparticle?

I don't understand which is the mechanism behind the visual reaction? I mean how the thiolated probe, the gold nanoparticle and the genomic DNA interact so that you see a color change?

04 May 2015 1,536 4 View

What is the difference between using water and acetic acid when making chitosan particles?

with which group of the chitosan reacts the acetic acid?

03 April 2015 8,122 14 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Italicization in molecule name: N-acetyl-p-benzoquinoneimine (NAPQI)?

Is there a convention for correct italicization of abbreviated parts of chemical molecule names? For...

13 January 2021 7,396 3 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View