What does the prefix "let" (for example, in let-7)in microRNAs nomenclature means?
The title's paper is: Development and validation of a rapid and reliable method for TPMT genotyping using real-time PCR. Author: Lorenz et al.2012. Clin Lab. 58(9-10):959-71 Thanks everyone!
03 April 2018 998 1 View
Studies in Logic and the Foundations of Mathematics Volume 25, 1959, Pages 31-51 Chapter 2 Aristotle's Theory of Science
03 April 2018 900 2 View
I've been reading a paper where the authors said this: The strong polyanionic nature of DNA increases the ionization of a polycation such as chitosan by reducing its surfacepotential and thereby...
02 March 2016 5,532 0 View
Which is better hydrochloric acid or acetic acid?why?
09 October 2015 611 3 View
How much volume I have to add of acetic acid and grams of sodium acetate?
09 October 2015 9,911 1 View
I saw this in a paper and I wanna do the same, but I don't know how much i have to add of DNA and EtBr: A solution of linear calf thymus DNA (10 µg/mL) in the appropriate buffer was mixed...
08 September 2015 7,296 0 View
I'm making experiments with chitosan particles to capture DNA. I have read that in an acidic medium chitosan is in it's protonate state so the interaction between the DNA and the chitosan it's...
08 September 2015 7,971 3 View
this is a reaction with citrate modified gold nanoparticles,thiolated probes and genomic DNA. First, i'm not sure for what reason you use thiolated probes, and second which is the mechanism behind...
04 May 2015 4,067 3 View
I don't understand which is the mechanism behind the visual reaction? I mean how the thiolated probe, the gold nanoparticle and the genomic DNA interact so that you see a color change?
04 May 2015 1,591 4 View
with which group of the chitosan reacts the acetic acid?
03 April 2015 8,187 14 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
the molecule name is citated in old literature about organic sunscreen molecules
10 July 2024 5,576 2 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
Article 87 of the Code declares all translated versions of the Code to be "equivalent in force, meaning and authority." I'm curious about what happens if there is a difference in meaning between...
22 May 2024 2,911 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View