09 September 2013 1 6K Report

Is there a way for enhancing the yield of extraction?

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Peptide lost during reverse phase HPLC?

Hello; I've dissolved my synthetic peptide in pure acetic acid and tried to purify it with reverse phase HPLC. I got weak peaks during analysis, however I injected at least 5 mg of my peptide!...

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How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

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Any protocol for solution based ELISA?

Hello; I've isolated peptides against PDL-1 from a phage library, but I can't confirm their binding to target in a solid based ELISA assay. So I want to check whether if I can get a positive...

04 May 2018 2,071 3 View

What is the easiest way to remove SDS following protein purification from gel?

I've purified my recombinant protein from SDS polyacrylamide gel and now I need to remove SDS for further work. Is it possible to do a buffer exchange on amicon filters? could anyone suggest any...

01 February 2017 1,286 4 View

Does anyone know how to justify the sample size in animal studies?

Hi there; I want to investigate anti tumor effects of an antagonist in murine models and I need to know how to determine number of animals in each group such that the results would be...

11 December 2016 1,265 9 View

What does the last number in cell line name mean?

Hi there; Does anyone one know what's the difference for example between SK-MEL-28 and SK-MEL-37? what does that number mean?

11 December 2016 3,686 0 View

Is there any way to induce tumorogenesis in murine models using human cell lines?

Hi there; I need to evaluate an anti cancer vaccine against a human protein in murine models.So I should use human cell lines. Is there any convenient way to induce tumorogenesis in murine models...

09 October 2016 1,989 3 View

Is there any convinient assay for in vivo gene expression evaluation?

Hi there; I want to needle inject Balb/C mouse with a plasmid coding for a specific gene and also GFP as a reporter and I need a method for in vivo gene expression assay! Is there any special kit...

06 July 2016 3,675 3 View

Why it's optimal for industrial microorganisms to grow in high temperature?

Hi there; I've read somewhere that growing in high temperatures is an advantage for industrial microorganisms; but unfortunately I couldn't find any reason for this in related articles! Is there...

04 May 2016 3,122 5 View

How can I detect secretary GFP-fused proteins in DMEM supernatant?

I've transfected HEK293-T cells with plasmid expressing for a secretary GFP-fused protein. I would like to know is there any method rather than ELISA test to detect my protein in supernatant using...

04 May 2015 4,773 4 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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