Raheleh Tooyserkani

21 Questions 28 Answers 0 Followers

Questions related from Raheleh Tooyserkani

Peptide lost during reverse phase HPLC?

Hello; I've dissolved my synthetic peptide in pure acetic acid and tried to purify it with reverse phase HPLC. I got weak peaks during analysis, however I injected at least 5 mg of my peptide!...

03 March 2019 9,569 5 View

How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

12 December 2018 7,194 2 View

Any protocol for solution based ELISA?

Hello; I've isolated peptides against PDL-1 from a phage library, but I can't confirm their binding to target in a solid based ELISA assay. So I want to check whether if I can get a positive...

05 May 2018 2,034 3 View

What is the easiest way to remove SDS following protein purification from gel?

I've purified my recombinant protein from SDS polyacrylamide gel and now I need to remove SDS for further work. Is it possible to do a buffer exchange on amicon filters? could anyone suggest any...

02 February 2017 1,234 4 View

What does the last number in cell line name mean?

Hi there; Does anyone one know what's the difference for example between SK-MEL-28 and SK-MEL-37? what does that number mean?

12 December 2016 3,645 0 View

Does anyone know how to justify the sample size in animal studies?

Hi there; I want to investigate anti tumor effects of an antagonist in murine models and I need to know how to determine number of animals in each group such that the results would be...

12 December 2016 1,227 9 View

Is there any way to induce tumorogenesis in murine models using human cell lines?

Hi there; I need to evaluate an anti cancer vaccine against a human protein in murine models.So I should use human cell lines. Is there any convenient way to induce tumorogenesis in murine models...

10 October 2016 1,943 3 View

Is there any convinient assay for in vivo gene expression evaluation?

Hi there; I want to needle inject Balb/C mouse with a plasmid coding for a specific gene and also GFP as a reporter and I need a method for in vivo gene expression assay! Is there any special kit...

07 July 2016 3,639 3 View

Why it's optimal for industrial microorganisms to grow in high temperature?

Hi there; I've read somewhere that growing in high temperatures is an advantage for industrial microorganisms; but unfortunately I couldn't find any reason for this in related articles! Is there...

05 May 2016 3,083 5 View

How can I detect secretary GFP-fused proteins in DMEM supernatant?

I've transfected HEK293-T cells with plasmid expressing for a secretary GFP-fused protein. I would like to know is there any method rather than ELISA test to detect my protein in supernatant using...

05 May 2015 4,728 4 View

What is the optimal concentration of G418 for selection of HEK293-T cells?

I've transfected HEK293-T cells with pCI as a mammalian expression vector containing neomycin gene as a selectable marker. I need to know the most efficient concentration of G418 for cell...

04 April 2015 2,388 5 View

Is it possible to use proteins eluted from SDS-gel for an antibody ELISA test?

I want to elute my protein from SDS-PAGE gel and coat it as target antigen in ELISA plate to consider the presence of antibody in rabbit serum.I need to know is it possible that the denatured...

02 February 2015 7,638 17 View

Is there any simple method to evaluate immune responses in immunized animals?

I have immunized rabbits and mice with DNA and I got no antibody answer in ELISA test! I need to know is there any other method to consider the presence of immune response?! I mean I want to know...

02 February 2015 5,944 6 View

Sudden stop in recombinant protein expression in BL21?

I have cloned a 25KD fragment in pet32a and I got recombinant expression in BL21. The yield of expression was very good, but suddenly it stopped expressing!I tried transforming new BL21 bacteria...

02 February 2015 4,695 2 View

Is Tolerance possible in DNA vaccination?

I'm working on polyclonal antibody production through genetic immunization in rabbits and after 5 month of immunization I got nothing! I need to know is there a problem with Tolerance induction...

01 January 2015 934 5 View

Call for participation in New Science/AAAS Webinar

Unearthing Hidden Genomic Data in Solid Tumor Samples: Are Your FFPE Samples Revealing All? Wednesday, September 18, 2013, at 9 a.m. Pacific, 12 noon Eastern, 5 p.m. UK, 6 p.m. CEST Learn from our...

09 September 2013 10,026 0 View

Why should we place bacterial cell on cold ice for 30 minutes, before performing plasmid extraction?

Is there a way for enhancing the yield of extraction?

09 September 2013 5,523 1 View

What is the exact sequence or structure difference between dimeric and tetrameric form of pyruvate kinase M2 in tumor cells?

Articles have mentioned they used antibodies to specifically detect the dimeric form but there's no sequence difference between dimer/tetramer as far as I know!

08 August 2013 4,076 2 View

Does anyone know about DNA electrotransfer?

I know that this works by a system similar to electroporation, but I need to use electrotransfer to deliver DNA to rabbit cells in vivo. I need more information about how I can exactly apply...

08 August 2013 9,074 0 View

Can anyone please suggest a comprehensive book on Methods?

I need to study more about common methods used in Biotechnology and proteomics like ELISA, chromatography, blotting. Does anyone know a useful book on these topics? Thanks

08 August 2013 6,204 7 View

Can anyone help regarding my problem designing cloning primers for a fusion sequence?

I'm designing cloning primers for a fusion sequence. I need the whole sequence from the first nucleotide to the end. Unfortunately the beginning and the end are highly GC rich so the Tm is somehow...

08 August 2013 4,073 9 View