Ganapati Bhat

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Questions related from Ganapati Bhat

Can someone help me regarding E. coli JM109 compatibility for Triparental mating?

I tried DH5 alpha and JM109 bacteria for transferring plasmid in to Agrobacterium tumefaciens EHA105 by tri-parental mating. I got a good response in DH5 alpha but not in JM109. Does anyone know...

07 July 2013 10,004 0 View

How can I eliminate pCAMBIA2301 from Agrobacterium tumefaciens EHA105?

I am trying to remove pCAMBIA2301 from EHA105. I tried to grow without kanamycin at 37C for several generation. but EHA105 still contains Kan resistance. Is there any alternative or and changes...

07 July 2013 3,431 2 View