I've purified my recombinant protein from SDS polyacrylamide gel and now I need to remove SDS for further work. Is it possible to do a buffer exchange on amicon filters?
could anyone suggest any other convenient method to remove SDS?
Thank you
- I used to routinely use Amicon filters to concentrate and alter diluent of purified protein
- If you like I can send you my old SOP when I return to work on Monday
When you recover your protein from an SDS PA gel, are you sure the protein is still in its native conformation and is not already denatured? In the case you need it for analysis, you can modify the procedure to recover protein from a gel for MS analysis. dissect the gel into small pieces and dehydrate using acetonitrile or use passive elution with buffer (see Thermofischer protocol to recover protein from gel).
You wont be able to completely remove sds from the protein using amicon filters or desalting columns.
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