Protein Expression of CAMKK2 is high in Monocyte, but very low in peripheral blood nuclear cells.how its differ?
Are you asking what is the difference between monocytes and PBMCs?
PBMCs contains lymphocytes (both Bs and Ts) and monocytes.
Also, what "monocyte" are you using? If you are using a cell line, they tend to differ from cell line to cell line.
@Shen-An Hwang thanks for your reply.Attached image is taken from Gene card for CAMKK2. In this expression level of protein in monocyte is high and in PBMC is very low.
Yes, but the image title also says in normal tissues and "cell lines". I'm assuming that the there is a different graph for cell lines?
And, my point is still valid, PBMC is a mixture of monocytes, T cells and B cells. Most are T cells, and then B cells and monocytes. Depending on the person the PBMC is isolated from, the T cell population can range between 50 to 70%. And since T cells, according to the figure provide, don't express CAMK2, then that will skew the entire PBMC population closer to reflect the T cell expression pattern than the monocyte expression pattern.
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