Sanger sequencing giving multiple peaks after few base pairs. What are the possible reasons? How can I confirm that? Suggest me way to get the seq ?

28 June 2023 1 10K Report

I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am attaching the chromatogram for your reference. We again re-sequenced them. Same results occurred after few clear peaks. Suggest me possible reasons for this and how can I confirm that.

Balaji B.

Does EtBr intercalation affect sanger sequencing?

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