I treated an Astrocytoma cell line to 10 gry of (gamma Irradiation), I noticed that an up-regulation of DNMT1, DNMT3a and DNMT3b gene expression. is there any explanation?
For example, gamma radiation (6 Gy) decreases mRNA expression of Kv, KATP and L-type Ca channels while down-regulates BKCa channels
Some publications argue for hypomethylation after irradiation, however that was done in different cell types. http://www.biomedcentral.com/1471-2164/16/56
or https://books.google.ru/books?id=Lw3ubsUQl1EC&pg=PA124&lpg=PA124&dq=DNMT+upregulation+radiation&source=bl&ots=RIz1S-qGzl&sig=-g9Nj7s7n2tqm1hIRKgbrcZDHAU&hl=en&sa=X&ved=0CC4Q6AEwAmoVChMIzrT21fmNxwIVBocsCh0RMAMN#v=onepage&q=DNMT%20upregulation%20radiation&f=false
But argue, if anything, for down regulation of DNTM expression after gamma.
Your effect could be a tissue or dose specific, of cause.
p38 and p53 are activated by gamma iradiation. It is possible that SWI/SNF histone remodeling complexes are modulated by PI3K/Akt, ERK/MAPK/MSK and p38 (Foster et al., 2006), (Pérez-Cadahía, 2011), (Simone et al., 2004). Transcription factors like STAT and NF-κB and c-Jun also interact with HAT/HDAC and DNMT1 (Icardi et al., 2012), (Ashburner, 2001), (Wolter et al., 2008). All epigenetic changes could in fact be activated by p38 a p53 as disscused in thease papers.
but the problem that i have an up-regulation of these genes after 10 gray of gamma radiation. many publications cited that there are a down regulation of these genes.
To find a clear explanation you have to provide more information; like, how about HDAC1 & 2? At what level of DNMTs expression you noticed? mRNA, protein or both?
How was the total experimental condition and set up?10 gray of gamma radiation generally ----
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