How I can create cancer of the lung of liver in rat?

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Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?

I'm trying to clone a ~900 bp gene which has a high GC ratio (about 75%) in E. coli. (Top10). The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if...

04 May 2016 7,304 7 View

Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector??

I'm trying to express a my target gene which has a high GC ratio about 75% in E. coli. The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if there are...

04 May 2016 1,168 1 View

Why is there an up-regulation on DNMT1, DNMT3a,DNMT3b gene expression after gamma irradiation?

I treated an Astrocytoma cell line to 10 gry of (gamma Irradiation), I noticed that an up-regulation of DNMT1, DNMT3a and DNMT3b gene expression. is there any explanation?

07 August 2015 4,229 5 View

How can I detect the membrane receptor of MDA-MB-231 for my extract?

I have a red seaweed extract, so I want to know which membrane receptor binds to my extract to activate the pathway?

04 May 2013 2,600 4 View

Full length NS5B gene amplification in HCV-4

I work with HCV-4, and I want to amplify the full lengh of the NS5B gene for the HCV4 type. Could anyone share the primer sequences and the PCR program?

05 June 2012 2,758 8 View

Do you know a target gene for growth hormone in human cell line?

I want to know the effects of growth hormone on human cell lines

04 May 2012 3,010 6 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Debottam Sinha

Sir...why dont u induce the transgene causing lung cancer...and create clones for better understanding and detection of da disease

Hossam Murad

But i dont have this transgene

Most probably...CCMB and CMC are working (hopefully) with the cell lines of mouse lung cancer cells.....

I sujjest u may contact them

Aleksandar Djordjevic

Maybe there is a specific chemical that causes cancer of the lungs, such as induction of rat colorectal cancer with 1,2-dimethyl-hydrazine (DMH).

Exactly....if the carcinogen is known then the cancer can be induced!!!!!! But it would be more cheap and less difficult to work with the cell lines than induction of the carcinogen and looking after the rats for around two weeks time!!!