Not sure what cell line you are using. If liver cell line, IGF-1 gene can be a target gene for GH.

i use MDA-231 and MCF7 and T98G

We use cultured skin fibroblasts and look at GH effects on both protein modification and gene expression. changes in gene expression are cell type specific and both time and dose dependent. Scan litersature and see which gene might work for your cell line.

I have no experience with these cell lines. You may have to check the literature.

These are breast cancer cell lines if I am not mistaken? I know they are IGF sensitive but is there downregulation of GH receptors?

Dear Andrew, you are in a good site, that exactly what i noticed when I treated theses cells line with GH