What should I choose for marking activated B cells?

02 March 2015 5,557 3 View

Are there any protocols for inducing immune tolerance to immunogenic protein in mice?

I am interested in immune tolerance. Are there any experimental protocols for incuding immune tolerance to an immunogenic protein? Thanks for your help!

07 August 2014 8,443 1 View

Why is chichen ovalbumin chosen as one of the most popular model antigen?

there are millions of proteins are immunogenic, however there are only several used as model antigens in immunological studies. One of the best known is chicken ovalbumin. Could someone tell me...

07 August 2014 7,893 11 View

Do RAG mice have more NK cells than normal ones?

I am going to isolate murine NK cells. I see some research papers using RAG mice to isolate the cells. Why? Is it because there are more NK cells in the spleens of RAG mice? And what's the percentage?

11 December 2013 5,832 5 View

Is there any way of generating feeder cells other than irradiation?

I'm going to culture NK cells. Some protocols say irradiated autologous or allogeneic feeder cells are needed. However, we don't have irradiators. Does it mean I can't get the feeder cells I need?...

08 September 2013 973 1 View

Is it possible that Hydrocortisone turn into crystals after stock frozen due to the high concentration?

08 September 2013 4,290 2 View

Are there any standards for choosing suitable antibodies for panning?

I'm doing panning to deplete T&B cells with CD3/CD19 antibodies. CD19 antibodies can remove B cells effectively, however, hamster anti-mouse CD3e can barely adsorb T cells. Both antibodies were...

08 September 2013 9,123 6 View

Can I use a different cell lines as negative or positive controls when doing flow cytometry?

When doing Flow Cytometry, I used B16 cells as negative control of IFN-gamma and Granzyme B. But apparently, the sizes of B16 and lymphocytes are so different. Is this allowed? Or, I can only use...

04 May 2013 4,140 4 View

How can I culture purified NK cells in vitro for a long time, for example 1-2 weeks?

I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...

03 April 2013 6,130 9 View

How can I remove dead cells from cultured lymphocytes with Percoll?

I'm using Percoll to remove dead cells from cultured lymphocytes, but I don't know which density gradients to use and which layers to collect. Can anyone give me some advice?

02 March 2013 2,191 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View