19 Questions 303 Answers 0 Followers
Questions related from Wolfgang Schechinger
What would you recommend? It should be cheap and of constant / defined composition, comparable to defatted milk powder (NFDM). Thanks!
01 January 2018 4,463 7 View
Dear Colleagues, I'm working on a recombinant protein (isolated from 3T3 mouse fibroblast supernatants) that behaves a little strange in analytics: Purified by IMAC (it has a His6-Tag) and gel...
12 December 2016 7,567 4 View
anyone knows by chance, which mammalian cellular structures it is staining? Just curious. Thanks!
06 June 2016 930 0 View
In homemade colloidal gel stains based on Coomassie Blue G250, 4..12% phosphoric acid are added as last step to promote colloid formation. How does it work: Is it "just" pH or do phosphate...
03 March 2016 10,073 2 View
Dear experts, I am planning a clean-up step in a protein purification process where I actually thought of using Sephacryl S200. In the stock room, I found some Sephadex G200 and thought, I'd give...
12 December 2015 9,869 6 View
Dear Colleagues, Any suggestions for alternatives to cyanoborohydride (in respect of less toxic, cheaper, suitable for aqueous settings)? It's about the reductive amination of a polysaccharide...
03 March 2015 7,003 10 View
Dear Colleagues, peptide synthesis companies offer different purities at different prices.What actually defines the purity? Several things come into my mind: - sequence errors (i.e. usually...
02 February 2015 4,535 7 View
Dear Colleagues, I happened to get a pretty soiled used centrifuge of this type and simply can't get the rotor out for a thorough cleaning of the machine. Of course, the screw has been removed and...
01 January 2015 5,918 9 View
Dear Colleagues, I'm in search of a ELISA to detect Glucagon Like Peptide 1 (GLP-1) in tissue culture supernatants and serum. Requirements: - detects total GLP-1 (active and inactive, N-terminal...
11 November 2014 9,526 2 View
Dear colleagues, Has anyone got a recipe for said buffer/solution to share? I have a secondary antibody conjugated to HRP that I'd like to store at higher dilution in the fridge/freezer. Probably...
08 August 2014 5,792 6 View
Dear colleagues, I have designed a construct for eukaryotic expression. Before I submit it for synthesis, I would like to find out about the possible implications of 3 (unintended) features that...
08 August 2014 3,381 1 View
I'm planning a gene synthesis project. The goal is to express the cDNA of a human-derived sequence in E.coli for protein production purposes. The company I submitted the sequence to has detected 2...
02 February 2014 9,092 9 View
I had the chance to obtain a Tecan 96PW microplate washer (washes 96 wells simultaneously). Of course, it's not the newest model (it's even 'phased out' already), but why not give it a second...
09 September 2013 1,582 4 View
I tried some chemical modifications on a primer sequence and tested them against the unmodified material. Actually, I just was about to check the ct value to see if they still were usable....
01 January 2013 3,875 3 View
Dear colleagues, I want to analyze some dsDNA (approx 110bp), but only have access to a protein gel acrylamide (AA) mix (30% AA in water, 37.5%:1% Acrylamide:bis-Acrylamide). Can I expect to see...
12 December 2012 4,220 2 View
When testing sputum samples with ELISA, instead of using DTT, which will destroy the antibody when the sample is applied to a antibody-coated ELISA-plate, is a solution to reduce the sputum...
11 November 2012 7,843 3 View
I am about to design a synthetic gene that includes the possibility of attaching a sequence for a polylinker (MCS, multiple cloning site). It will be cloned into a (E. coli expression-) vector...
11 November 2012 6,143 6 View
I am planning some synthetic genes to express human sequences (an inactive pro-protease and a structural protein, both with a His and a myc tag) in E. coli for purification, I am open for...
11 November 2012 487 14 View
Does anyone have a good resource to share on describing the colorimetric signal in sandwich ELISA mathematically as a function of antibody- and antigen concentration and other variables? In order...
10 October 2012 2,792 7 View
