Is there any way of generating feeder cells other than irradiation?

09 September 2013 1 1K Report

I'm going to culture NK cells. Some protocols say irradiated autologous or allogeneic feeder cells are needed. However, we don't have irradiators. Does it mean I can't get the feeder cells I need? Is there any other equivalent way?

Damien G Harkin

The other standard method for growth-inactivation of feeder cells is treatment with mitomycin C.

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