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Questions related from Fei Yang
I want to distinguish activated B cells from NaÏve B cells in mouse. I've learned that, in humans, CD27 is the ideal marker. However, I'm not sure whether it's the same in the mice. If not, which...
03 March 2015 5,647 3 View
there are millions of proteins are immunogenic, however there are only several used as model antigens in immunological studies. One of the best known is chicken ovalbumin. Could someone tell me...
08 August 2014 6,703 2 View
08 August 2014 8,041 11 View
I am interested in immune tolerance. Are there any experimental protocols for incuding immune tolerance to an immunogenic protein? Thanks for your help!
08 August 2014 8,549 1 View
I am going to isolate murine NK cells. I see some research papers using RAG mice to isolate the cells. Why? Is it because there are more NK cells in the spleens of RAG mice? And what's the percentage?
12 December 2013 5,932 5 View
I'm going to culture NK cells. Some protocols say irradiated autologous or allogeneic feeder cells are needed. However, we don't have irradiators. Does it mean I can't get the feeder cells I need?...
09 September 2013 1,070 1 View
I'm using hydrocortisone to culture murine cells. The stock I use is 50 μg/ml. I store it at -20°C in small aliquots. However, I often found crystals in the media under microscope. Has anyone had...
09 September 2013 4,386 2 View
I'm doing panning to deplete T&B cells with CD3/CD19 antibodies. CD19 antibodies can remove B cells effectively, however, hamster anti-mouse CD3e can barely adsorb T cells. Both antibodies were...
09 September 2013 9,218 6 View
When doing Flow Cytometry, I used B16 cells as negative control of IFN-gamma and Granzyme B. But apparently, the sizes of B16 and lymphocytes are so different. Is this allowed? Or, I can only use...
05 May 2013 4,244 4 View
I'm culturing murine NK cells in vitro. However, the second day post isolation, 80% or more of the cells are dead or apoptotic. The medium I used was a-MEM supplemented with 10% FBS, 10 ng/ml...
04 April 2013 6,395 9 View
I'm using Percoll to remove dead cells from cultured lymphocytes, but I don't know which density gradients to use and which layers to collect. Can anyone give me some advice?
03 March 2013 2,352 3 View
I want to visualize murine blood vessels in vivo, but fluorescent dye-conjugated dextrans are too expensive. Is there a less expensive way?
02 February 2013 8,538 0 View
I'm reading a paper about ADCC. The authors used rat antibodies to coat tumor cells. But the NK cells they used were from mice. Can murine FcR recognize rat antibody Fc ends? I don't know. Could...
02 February 2013 9,281 3 View
I want to deplete some subpopulations of immune cells from bone marrow with petri dish precoated with antibodies. But I don't know how to make it and how much antibody I need. Can anyone who has...
01 January 2013 1,872 19 View
