17 Questions 5 Answers 0 Followers
Questions related from Kiriakos Athanasiadis
I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...
12 June 2024 673 1 View
So, i m working during last month with GC-MS. When i'm making the extraction of samples e.g. PAH samples, i'm pipetting significant concentration of two internal controls into the solution. When i...
01 April 2024 6,518 1 View
I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...
25 March 2024 5,611 3 View
I have 4 substances for PAH, each one is 100ppm or 1000ppm, i would like to make a mix(4-PAH) of them of final volume 1mL and final concentration 100μg/kg. How can i proceed? Thank you in advance,
12 March 2024 5,539 2 View
Hallo! I m working on GC-MS instrument these days and i am installing some methods. I would like to ask if you know any research paper or directly a method for Dioxins/Furans via GC-MS?
07 March 2024 7,721 2 View
I am using PCR Instrument called SLAN from HONGSHI. Any clue?
24 January 2024 9,673 7 View
I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?
18 January 2024 1,397 2 View
I m gonna make an excel for results interpretation for gene expression on food matrixes for quantification. How can i proceed? Thanks you in advance, Kiriakos
04 December 2023 5,774 0 View
Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos
04 December 2023 1,758 2 View
I have 10mg/kg obtained by using HPLC, How to convert to Calcium citrate? Thank you,
13 October 2023 4,286 3 View
I am running via ELISA method Dioxins/furans analysis for food matrixes and i would like to ask if there is any PCR technique for quantitative determination. Thank you in advance,
12 October 2023 3,607 1 View
We re facing a troubleshooting working with olive paste as a matrix. We re trying to extract using Macherey Nagel DNA extraction kit for food matrixes and the yield, purity and DNA concentration...
22 September 2023 3,988 0 View
So, i would like to know what concentration of Tris HCl i have to prepare in order to use it in RNA isolation protocol? Additionally, i would like to know why to use this buffer, is it only for pH...
25 July 2023 5,483 3 View
I would like to isolate RNA from plants, is an low cost RNA isolation protocol available? Preferable using Chloroform as main reagent. Thank you in advance, Kiriakos Athanasiadis
25 July 2023 8,025 2 View
How can i calculate with precision the concentration of DNA/RNA aliquots in order to load a quantitative PCR?
06 July 2023 6,267 4 View
Hi, i m using Agilent HPLC and i m facing a troubleshooting with degasser, the led turns red when i m increasing the flow from 1 to 3 and finally to 5mL/min. Also, when the led is green, i see...
06 May 2023 9,610 6 View
Good afternoon, When we are facing a troubleshooting on GC-FID with standards. In big concentrations we dont have any trouble and all the Gauss type areas are on the proper retention time. After...
20 March 2023 2,558 1 View
