Kiriakos Athanasiadis

24 Questions 9 Answers 0 Followers

Questions related from Kiriakos Athanasiadis

Any Kit for Sources of Gluten?

I am searching a commercial Molecular Biology RT-PCR for qualitative/quantitative determination of gluten source(Rye, Wheat and Barley mostly). Any clue? Thank you in advance, Kiriakos

21 May 2025 3,224 2 View

Questions about Illumina HiSeq 4000?

So, I have obtained Illumina HiSeq 4000 instrument. So, I have a request about PE100 Sequencing. Does anyone is using currently or used before this sequencer because I have a lot questions the...

14 May 2025 9,153 0 View

Questions about E.coli O157:H7?

I have obtained 4000cfu standard pellet and I have made serial dilutions to reach 3-5cfu, so I m working with Sorbitol McConkey agar. But as I proceed to the dilutions to reach the desired level,...

14 May 2025 3,785 7 View

Why Internal control is coming earlier than my sample at RT-PCR?

So my Internal control is coming at 21.5 and my sample to 26.5 Cq. So, i have my standard curves and I make my calculations and is resulting very high value about my sample(Something about 145%...

25 April 2025 8,060 1 View

Anyone familiar with Agilent Aria Dx, Real-Time PCR System?

Hallo there! I am facing several troubleshootings with Agilent Aria Dx Real-Time PCR System. So, there are below: 1. ID 301-Motor Error 2. ID 269-Motion Error 3. ID 269-Motion Error 4. ID...

16 September 2024 7,726 0 View

Ordering Insects for experiments?

Hallo! I would like to order insects for my experiments about my PhD for molecular biology experiments in particular. Do you have any recommendations for a website to get in touch? Thank you...

04 July 2024 4,517 0 View

Need to obtain concentration of DNA/RNA?

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...

12 June 2024 771 1 View

I have some questions for calculation working with GC MS, help?

So, i m working during last month with GC-MS. When i'm making the extraction of samples e.g. PAH samples, i'm pipetting significant concentration of two internal controls into the solution. When i...

01 April 2024 6,589 1 View

How can i transform copies DNA to mg/kg?

I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...

25 March 2024 5,725 3 View

Question about calculation for Standards GC-MS?

I have 4 substances for PAH, each one is 100ppm or 1000ppm, i would like to make a mix(4-PAH) of them of final volume 1mL and final concentration 100μg/kg. How can i proceed? Thank you in advance,

12 March 2024 5,713 2 View

Determination of Dioxins/Furans via GC-MS?

Hallo! I m working on GC-MS instrument these days and i am installing some methods. I would like to ask if you know any research paper or directly a method for Dioxins/Furans via GC-MS?

07 March 2024 7,896 2 View

I am using BIO0-RAD Tubes and Caps for my PCR Reactions, so when PCR starts the tubes are opened (95Celsius). What i have to do?

I am using PCR Instrument called SLAN from HONGSHI. Any clue?

24 January 2024 9,774 7 View

May i use knock out gene method to obtain confirmation for gene expression?

I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?

18 January 2024 1,490 2 View

What i have to pay attention in gene expression interpretation?

I m gonna make an excel for results interpretation for gene expression on food matrixes for quantification. How can i proceed? Thanks you in advance, Kiriakos

04 December 2023 5,862 0 View

May i use Northern blot to ensure my RT-PCR results?

Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos

04 December 2023 1,845 2 View

How to convert Citric acid to Calcium citrate?

I have 10mg/kg obtained by using HPLC, How to convert to Calcium citrate? Thank you,

13 October 2023 4,376 3 View

PCR Technique for Dioxins/furans quantitative detection?

I am running via ELISA method Dioxins/furans analysis for food matrixes and i would like to ask if there is any PCR technique for quantitative determination. Thank you in advance,

12 October 2023 3,684 1 View

Troubleshooting with olive paste DNA extraction. Any solution?

We re facing a troubleshooting working with olive paste as a matrix. We re trying to extract using Macherey Nagel DNA extraction kit for food matrixes and the yield, purity and DNA concentration...

22 September 2023 4,061 0 View

Why to use Tris HCl buffer for RNA isolation?

So, i would like to know what concentration of Tris HCl i have to prepare in order to use it in RNA isolation protocol? Additionally, i would like to know why to use this buffer, is it only for pH...

25 July 2023 5,559 3 View

Is any low cost RNA isolation protocol available?

I would like to isolate RNA from plants, is an low cost RNA isolation protocol available? Preferable using Chloroform as main reagent. Thank you in advance, Kiriakos Athanasiadis

25 July 2023 8,099 2 View

Calculation for concentration and other parameters for DNA/RNA aliquots?

How can i calculate with precision the concentration of DNA/RNA aliquots in order to load a quantitative PCR?

06 July 2023 6,326 4 View

A help for troubleshooting with degasser?

Hi, i m using Agilent HPLC and i m facing a troubleshooting with degasser, the led turns red when i m increasing the flow from 1 to 3 and finally to 5mL/min. Also, when the led is green, i see...

06 May 2023 9,668 6 View

Troubleshooting with standards on GC-ECD?

Good afternoon, When we are facing a troubleshooting on GC-FID with standards. In big concentrations we dont have any trouble and all the Gauss type areas are on the proper retention time. After...

20 March 2023 2,615 1 View

How can i break efficiently Chlamydomonas reinhardtii cells for metabolites and enzyme activity determination?

I have done sonication but i faced some problems, for instance the alteration of temperature vs time.

23 April 2018 9,802 2 View