How to convert Citric acid to Calcium citrate?

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Need to obtain concentration of DNA/RNA?

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...

11 June 2024 712 1 View

I have some questions for calculation working with GC MS, help?

So, i m working during last month with GC-MS. When i'm making the extraction of samples e.g. PAH samples, i'm pipetting significant concentration of two internal controls into the solution. When i...

31 March 2024 6,551 1 View

How can i transform copies DNA to mg/kg?

I am working with an allergen and i am working using PCR, the result that offers the kit is copies DNA, although i need to give a result in mg/kg. Is any possible way? Thank you in advance,...

24 March 2024 5,657 3 View

Question about calculation for Standards GC-MS?

I have 4 substances for PAH, each one is 100ppm or 1000ppm, i would like to make a mix(4-PAH) of them of final volume 1mL and final concentration 100μg/kg. How can i proceed? Thank you in advance,

11 March 2024 5,660 2 View

Determination of Dioxins/Furans via GC-MS?

Hallo! I m working on GC-MS instrument these days and i am installing some methods. I would like to ask if you know any research paper or directly a method for Dioxins/Furans via GC-MS?

06 March 2024 7,843 2 View

I am using BIO0-RAD Tubes and Caps for my PCR Reactions, so when PCR starts the tubes are opened (95Celsius). What i have to do?

I am using PCR Instrument called SLAN from HONGSHI. Any clue?

23 January 2024 9,713 7 View

May i use knock out gene method to obtain confirmation for gene expression?

I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?

17 January 2024 1,445 2 View

May i use Northern blot to ensure my RT-PCR results?

Is it highly recommended to use northern blot to ensure my RT-PCR results instead of LC-MS for example? Thank you in advance, Kiriakos

03 December 2023 1,794 2 View

What i have to pay attention in gene expression interpretation?

I m gonna make an excel for results interpretation for gene expression on food matrixes for quantification. How can i proceed? Thanks you in advance, Kiriakos

03 December 2023 5,814 0 View

PCR Technique for Dioxins/furans quantitative detection?

I am running via ELISA method Dioxins/furans analysis for food matrixes and i would like to ask if there is any PCR technique for quantitative determination. Thank you in advance,

11 October 2023 3,638 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,800 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,622 4 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,491 0 View