Why dont we use Ninhydrin as a developer rather than commassie brilliant blue in SDS PAGE?

More Muneeb Faiq's questions See All

Is "thinking" an act of science?

When we think, what do we do? Do we philosophize or exercise science? Neither, either or both? How is thinking not science and how is thinking an act of science? How is it philosophy and how not a...

06 July 2019 1,268 5 View

What is theoretical Medicine?

Why cant there be a journal dedicated to the field of medicine which does not yet have any experimental basis much like theoretical medicine?

06 July 2019 1,238 9 View

Where can we submit/publish general philosophy essays?

There are limited places/portals/magazines that publish unsolicited general philosophy essays. Could anyone guide me how can I get my essays published on regular basis? Thank you

06 July 2019 6,326 2 View

How is artificial intelligence same or different from your intelligence?

How is artificial intelligence same or different from what we conventionally call natural intelligence?

06 July 2019 3,336 6 View

What does it mean to exist?

What does it actually mean to exist? How is it different from non-existence? How can we be sure if something exists or does not exist? I have made a case that these questions are more fundamental...

04 May 2019 5,109 24 View

Is slight illness is a good thing?

Are there any uses of illness or it is just all evil?

07 August 2018 3,086 1 View

Does "Sense of Humor" play any role in health and disease?

07 August 2018 4,519 5 View

How to publish a hypothesis without experimental data?

05 June 2018 5,196 8 View

Why is Mesentery considered an Organ?

Though Mesentery may be considered as a single entity all along the gut tube, but why is it called an organ? I don't see any reason for that. Neither is there any international body to come up...

05 June 2018 5,437 37 View

Difference between Dye and Stain?

What is the difference between Dye and Stain in Biological Experiments?

05 June 2018 995 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Soner Öner-Sieben

Article A modified method for amino acid analysis with ninhydrin of ...

https://link.springer.com/article/10.1007%2Fs00726-013-1513-1

Dominique Liger

Hi there,

SDS/PAGE is prepared with Tris which contains a primary amine which would react with ninhydrin so staining would be uniform and intense.