Ninhydrin stains aminoacids rapidly. Why dont we use Ninhydrin as a developer rather than commassie brilliant blue in SDS PAGE? wont that save time?
Article A modified method for amino acid analysis with ninhydrin of ...
https://link.springer.com/article/10.1007%2Fs00726-013-1513-1
Thanks Soner!
I will go through these papers and get back soon.
Best wishes
Hi there,
SDS/PAGE is prepared with Tris which contains a primary amine which would react with ninhydrin so staining would be uniform and intense.
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