Why dont we use Ninhydrin as a developer rather than commassie brilliant blue in SDS PAGE?

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Soner Öner-Sieben

Article A modified method for amino acid analysis with ninhydrin of ...

https://link.springer.com/article/10.1007%2Fs00726-013-1513-1

Muneeb Faiq

Thanks Soner!

I will go through these papers and get back soon.

Best wishes

Dominique Liger

Hi there,

SDS/PAGE is prepared with Tris which contains a primary amine which would react with ninhydrin so staining would be uniform and intense.