What is the difference between Dye and Stain in Biological Experiments?
What is the difference between stain and dye?
A stain is temporary whereas dyes are permanent and can be removed only after cell wall destruction
A dye is coloring agent used for general purposes and a stain is used for any biological specimen staining.
Also dye is crude and stain is purified form.
Image may helpful:
When we think, what do we do? Do we philosophize or exercise science? Neither, either or both? How is thinking not science and how is thinking an act of science? How is it philosophy and how not a...
06 July 2019 1,519 5 View
Why cant there be a journal dedicated to the field of medicine which does not yet have any experimental basis much like theoretical medicine?
06 July 2019 1,553 9 View
There are limited places/portals/magazines that publish unsolicited general philosophy essays. Could anyone guide me how can I get my essays published on regular basis? Thank you
06 July 2019 6,550 2 View
How is artificial intelligence same or different from what we conventionally call natural intelligence?
06 July 2019 3,558 6 View
What does it actually mean to exist? How is it different from non-existence? How can we be sure if something exists or does not exist? I have made a case that these questions are more fundamental...
04 May 2019 5,416 24 View
Does "Sense of Humor" play any role in health and disease?
07 August 2018 4,736 5 View
Are there any uses of illness or it is just all evil?
07 August 2018 3,386 1 View
How to publish a hypothesis without experimental data?
05 June 2018 5,443 8 View
http://www.timesnownews.com/health/article/psychological-stress-could-cause-blindness/243759
05 June 2018 5,065 6 View
Though Mesentery may be considered as a single entity all along the gut tube, but why is it called an organ? I don't see any reason for that. Neither is there any international body to come up...
05 June 2018 5,669 37 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View