Is "thinking" an act of science?

When we think, what do we do? Do we philosophize or exercise science? Neither, either or both? How is thinking not science and how is thinking an act of science? How is it philosophy and how not a...

06 July 2019 1,268 5 View

What is theoretical Medicine?

Why cant there be a journal dedicated to the field of medicine which does not yet have any experimental basis much like theoretical medicine?

06 July 2019 1,238 9 View

Where can we submit/publish general philosophy essays?

There are limited places/portals/magazines that publish unsolicited general philosophy essays. Could anyone guide me how can I get my essays published on regular basis? Thank you

06 July 2019 6,326 2 View

How is artificial intelligence same or different from your intelligence?

How is artificial intelligence same or different from what we conventionally call natural intelligence?

06 July 2019 3,336 6 View

What does it mean to exist?

What does it actually mean to exist? How is it different from non-existence? How can we be sure if something exists or does not exist? I have made a case that these questions are more fundamental...

04 May 2019 5,109 24 View

Is slight illness is a good thing?

Are there any uses of illness or it is just all evil?

07 August 2018 3,086 1 View

Does "Sense of Humor" play any role in health and disease?

Does "Sense of Humor" play any role in health and disease?

07 August 2018 4,519 5 View

How to publish a hypothesis without experimental data?

How to publish a hypothesis without experimental data?

05 June 2018 5,196 8 View

Why is Mesentery considered an Organ?

Though Mesentery may be considered as a single entity all along the gut tube, but why is it called an organ? I don't see any reason for that. Neither is there any international body to come up...

05 June 2018 5,437 37 View

Stress can cause vision loss?

http://www.timesnownews.com/health/article/psychological-stress-could-cause-blindness/243759

05 June 2018 4,864 6 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View