Hania Elhoshy

8 Questions 7 Answers 0 Followers

Questions related from Hania Elhoshy

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

29 July 2024 2,495 1 View

Why are my astrocyte cultures contaminated with alot of microglia and opc?

Hello Everyone ! I isolated mixed cells from neonatal mouse cortices , and they are always contaminated with other cells (by imaging it appears that my astrocytes are even the least common...

14 November 2023 480 0 View

ROS measurement by Mitosox - Flow cytometry .. why does untreated grp show higher Fluorescence intensity than LPS group ?

could anyone please help me figure out what I could be doing wrong ? how could the untreated group show higher ROS than LPS grp ? I am using primary astrocytes , treating with 1 micogram per ml...

09 November 2023 8,352 4 View

Morris Water Maze : Why would mice jump off the platform after reaching it ?

I am trying to conduct the Morris Water Maze , but it seems that the mice do not want to reach the platform. what would be a possibe cause ? when they reach the platform they sometimes stand on...

27 March 2023 3,264 0 View

Morris Water Maze : Why would mice jump off the platform after reaching it ?

I am trying to conduct the Morris Water Maze , but it seems that the mice do not want to reach the platform. what would be a possibe cause ? when they reach the platform they sometimes stand on it...

01 January 1970 5,974 4 View

Why are my astrocyte cultures contaminated with alot of microglia and opc?

Hello Everyone ! I isolated mixed cells from neonatal mouse cortices , and they are always contaminated with other cells (by imaging it appears that my astrocytes are even the least common...

01 January 1970 7,311 2 View

Does treating cells with several compounds affect their viability? (eg: inducer .. treatment .. modulator etc )?

Hello everyone , I am following a protocol where I treat my cells with 1 .. 2.. or 3 compounds and I do an atp cell titer glo assay I noticed that the more compounds my cells receive . the...

01 January 1970 6,693 4 View

ROS measurement by Mitosox - Flow cytometry .. why does untreated grp show higher Fluorescence intensity than LPS group ?

could anyone please help me figure out what I could be doing wrong ? how could the untreated group show higher ROS than LPS grp ? I am using primary astrocytes , treating with 1 micogram per ml...

01 January 1970 7,670 0 View