Can I use P-E Titan MPS microwave digester for sample preparation for Kjeldahl nitrogen determination?

More Jakub Kostecki's questions See All

7-Azido-4-Methylcoumarin (7-AzidoMC) as a fluorescent probe for detecting hydrogen sulfide (H2S)?

How effective is 7-Azido-4-Methylcoumarin (7-AzidoMC) as a fluorescent probe for detecting hydrogen sulfide (H2S) in various brain samples such as cell plasma, tissue homogenates, and stained...

03 April 2024 5,389 0 View

What tools are currently available to predict operons in bacterial genomes?

Hi everyone, I was looking for some tools (preferably web-based tools) to predict genes organized in operons in various bacterial sequences I currently work on (plasmids, chromosoms, etc.). For...

19 February 2024 8,922 2 View

What are the recepies for Aerogel synthesis?

I am looking for new recepie /formulas for Aerogels synthesis, and new cooperation in this field.

31 December 2023 8,121 0 View

Why does viability in chondrocytes not decrease after treatment with LPS?

Hello, I have been exposing human chondrocytes to LPS (Lipopolysaccharides from E. coli O111:B4) in concentrations from 1 µg/ml to 40 µg/ml for 24 h and 48 h and haven’t seen any decrease in cell...

16 November 2023 5,297 2 View

Why during my compression tests the stress-strain curve is concave?

I was doing the compression tests on previously thermomechanically deformed pure titanium. The samples were uniaxially compressed in room temperature. The samples were cylindrical, and their...

04 October 2023 6,826 1 View

What is the state of art of the machine learning and AI in the design of experiment?

Is the machine learning and AI in the experimental design a thing?

25 June 2023 4,822 2 View

Are there scales, check lists or other methods to assess the quality and transparency of research using patient data (especially imaging)?

Are there methods to evaluate studies using medical data?

06 June 2023 9,384 3 View

How to measure the Minimal Detectable Change?

Hello, at this moment I am doing an literature overview in topic of clinical studies about viscosupplementation in OA patients. I am interested in the possibilities to count the Minimal...

01 March 2023 2,486 3 View

Is knowledge of biological age essential for strength training in soccer during puberty or can we train safely without this information?

How can information about the biological age of elite players be used in strength training during puberty? Can strength training be individualized with respect to the peak high velocity? Can...

28 January 2023 3,875 3 View

How do you deal with cytotoxicity in a factorial design?

Hello. I would like to ask, how should I incorporate the cytotoxicity as a response in the factorial design, since cytotoxicity depends on concentration? My only idea is to evaluate the IC50 for...

04 December 2022 8,045 2 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

What additional processing or treatment options are available to enhance biogas plant digestate quality or value?

One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...

20 July 2024 3,392 2 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

From different feedstocks based Biogas digesters what would be the standard NPK - Nutrient contents of the digestate, and how consistent is it?

One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...

18 July 2024 9,907 12 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View