I have a prolem with my PCR products. For the fist time I used PCR then tooke the products on gel electrophoresis, with the published protocol, my results became positive. For the next time the same PCR products with same protocols and samples were negative on gel electrophoresis. I am a M.Sc student and don't know what should I do. If there is someone who had the same problesms it would be great to help me.

Thank you

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