12 December 2019 9 7K Report

Hello,

Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile phase, I got two big negative peaks which are repeatable. Wired to me. I am sure the instrument is all right and no air injected.

Is it possible that any components are fluorescent in the sample which could be excited on UV 220nm?

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