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01 February 2020 5,449 3 View

Why did I get negative peaks in HPLC with a UV detector and a SEC column?

Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...

11 December 2019 6,709 9 View

How to isolate peptides from sugar solution?

Dear all, I try to isolate peptide (MW less than 3000Da ) from sugar solution (sugar concentration ~30%). The concentration of the peptide might be very low, but it is ok if the isolated peptides...

31 December 2018 4,261 3 View

What is the white grains on the leaves, wax secretion?

Hello, I saw many white grains on the leaves of my Hibiscus plants in the green house. Does any people know what is it? It doesn't look like any insects' egg. And it can't dissolve in water. Wax? Zha

08 September 2018 2,532 0 View

Is it possible that L-gulonolactone oxidase diminish hydrogen peroxide by using ascorbate and produce L-gulono-1,4-lactone and oxygen?

Dear Colleagues We know that L-gulonolactone oxidase catalyzes the reaction of L-gulono-1,4-lactone with oxygen to L-xylo-hex-3-gulonolactone and hydrogen peroxide. Is this reaction reversible? Or...

04 May 2017 3,537 0 View

How to purify FAD-dependent enzyme?

Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha

03 April 2017 5,107 9 View

How to quantify FAD in biological samples?

Dear Colleagues, I am looking for a method that could be used to measure FAD concentration (Flavin adenine dinucleotide) in biological samples, such as nectar or plant bleeding sap. Hope any...

02 March 2017 5,016 2 View

Why are monocots and dicots mixed in the phylogeny tree?

04 May 2016 3,045 10 View

How many times can Tenax TA column be used for floral scent analysis?

10 November 2014 2,900 3 View

Can someone help with identifying this beetle (see attached)?

Can anyone help with identifying this beetle? It is less than 0.5 cm long. The photo was taken in China.

08 September 2014 2,703 8 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View