05 May 2016 10 3K Report

Dear colleagues,

My colleague and I cloned a gene from sword bean which is mainly expressed in flower. After I did blast using the protein's amino acid sequence, I found there are just 250 homologues in the database (Uniprot), and only exist in angiosperms including the basal species  Amborella trichopoda.

Then, I used the amino acids sequence of these proteins built the

phylogeny tree as attached, and placed Amborella trichopoda at root.The tree (NJ) looks a little strange,  the monocots mixed with dicots. In fact, when using NA sequences to generate the tree, the tree shapes are similar. 

My questions are :

1. Because no homologues could be found in other than angiosperms.

Can I say this protein appear very later along with evolution of angiosperm? 

2. Can I say this protein evolves slowly or conservative, the 250 proteins from angiosperms range from 260-300 AAs and few subtypes of this protein had been reported ?  

3. Convergent evolution? Monocots and dicots mixed in the tree.

 

regards

 Zha

More H.G. Zha's questions See All
Similar questions and discussions