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01 February 2020 5,449 3 View

Why did I get negative peaks in HPLC with a UV detector and a SEC column?

Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...

11 December 2019 6,709 9 View

How to isolate peptides from sugar solution?

Dear all, I try to isolate peptide (MW less than 3000Da ) from sugar solution (sugar concentration ~30%). The concentration of the peptide might be very low, but it is ok if the isolated peptides...

31 December 2018 4,261 3 View

What is the white grains on the leaves, wax secretion?

Hello, I saw many white grains on the leaves of my Hibiscus plants in the green house. Does any people know what is it? It doesn't look like any insects' egg. And it can't dissolve in water. Wax? Zha

08 September 2018 2,532 0 View

How to purify FAD-dependent enzyme?

Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha

03 April 2017 5,107 9 View

How to quantify FAD in biological samples?

Dear Colleagues, I am looking for a method that could be used to measure FAD concentration (Flavin adenine dinucleotide) in biological samples, such as nectar or plant bleeding sap. Hope any...

02 March 2017 5,016 2 View

Why are monocots and dicots mixed in the phylogeny tree?

04 May 2016 3,045 10 View

Please help to identify this plant.

Please help to identify this plant. I found it in a green house. A species from Liliaceae, but not sure about the exact name. Please help to identify it.

03 April 2016 3,333 10 View

How many times can Tenax TA column be used for floral scent analysis?

10 November 2014 2,900 3 View

Can someone help with identifying this beetle (see attached)?

Can anyone help with identifying this beetle? It is less than 0.5 cm long. The photo was taken in China.

08 September 2014 2,703 8 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View