14 Questions 36 Answers 0 Followers
Questions related from H.G. Zha
Hello, Does anyone know where to buy the ‘Curdlan-Remazol Brilliant Blue (CM-Curdlan-RBB)“ for beta 1,3-glucanase activity assay? It looks had been offered by "Loewe Biochemica GmbH", but I...
02 February 2020 5,587 3 View
Hello, Today, when I analyzed my nectar sample (containing plant secondary metabolites, amino acids and proteins) using a HPLC with a UV detector (220nm) and a SEC column, NaOAC buffer as mobile...
12 December 2019 6,935 9 View
Dear all, I try to isolate peptide (MW less than 3000Da ) from sugar solution (sugar concentration ~30%). The concentration of the peptide might be very low, but it is ok if the isolated peptides...
01 January 2019 4,368 3 View
Hello, I saw many white grains on the leaves of my Hibiscus plants in the green house. Does any people know what is it? It doesn't look like any insects' egg. And it can't dissolve in water. Wax? Zha
09 September 2018 2,622 0 View
Dear Colleagues We know that L-gulonolactone oxidase catalyzes the reaction of L-gulono-1,4-lactone with oxygen to L-xylo-hex-3-gulonolactone and hydrogen peroxide. Is this reaction reversible? Or...
05 May 2017 3,634 0 View
Dear Colleagues I am trying to purify a FAD-binding protein. Is there a reliable method I can use to purity such proteins? Best Zha
04 April 2017 5,213 9 View
Dear Colleagues, I am looking for a method that could be used to measure FAD concentration (Flavin adenine dinucleotide) in biological samples, such as nectar or plant bleeding sap. Hope any...
03 March 2017 5,108 2 View
Dear colleagues, My colleague and I cloned a gene from sword bean which is mainly expressed in flower. After I did blast using the protein's amino acid sequence, I found there are just 250...
05 May 2016 3,163 10 View
Please help to identify this plant. I found it in a green house. A species from Liliaceae, but not sure about the exact name. Please help to identify it.
04 April 2016 3,437 10 View
I am using Tenax TA (0.1g per column) as adsorbent for my floral scent analysis. I bought Tenax TA from Sigma, prepare the column by myself and preform the experiment as suggested by Raguso et al...
11 November 2014 2,994 3 View
Can anyone help with identifying this beetle? It is less than 0.5 cm long. The photo was taken in China.
09 September 2014 2,794 8 View
I have watched pollinators pollinate my plants in the daytime, but how can I do it in the middle of the night? I dont think I can use a light otherwise unwanted insects may be attracted as well....
07 July 2013 6,789 0 View
I am looking for a simple method to measure the total alkaloid content in nectar without separation, such as Colorimetric method. My plant is a Solanaceae species. I noticed that some people use...
07 July 2013 3,515 7 View
The total concentration of peptides in the solution might be very low, less than 100ug/ml. I am not sure how many kinds of peptide are in the solution. I plan to identify them by MASS. I also...
06 June 2013 4,563 10 View
